Publications

Clees, A., Stolp, V., Häupl, B., Fuhrmann, D.C., Wempe, F., Seibert, M., Weber, S., Banning, A., Tikkanen, R., Williams, R., Brüne, B., Serve, H., Schnütgen, F.*, von Metzler, I.*, and Kurrle, N.* (2021) Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene. Cells, 11, 292 [Pubmed][Journal] 

Wilke, A., Doebele, C., Zindel, A., Lee, K.S., Rieke, S., Ceribelli, M., Comoglio, F., Phelan, J., Wang, J., Pikman, A., Jahn, D., Häupl, B., Schneider, C., Scheich, S., Tosto, F., Bohnenberger, H., Stauder, P., Schnütgen, F., Słabicki, M., Coulibaly, Z., Wolf, S., Bojarczuk, K., Chapuy, B., Brandts, B., Stroebel, P., Lewis, C., Engelke, M., Xu, X., Kim, H., Dang, T.H., Schmitz, R., Hodson, D.J., Stegmaier, K., Urlaub, H., Serve, H., Schmitt, C., Kreuz, F., Knittel, G., Rabinowitz, J., Reinhardt, H., Vander Heiden, M., Thomas, C., Staudt, L., Zenz, T., and Oellerich, T. (2021) SHMT2 inhibition disrupts the TCF3 TCF3 transcriptional survival program in Burkitt lymphoma. Blood [Pubmed][Journal] 

Makowka, P., Stolp, V., Stoschek, K., and Serve, H. (2021) Molecular determinants of therapy response of venetoclax-based combinations in acute myeloid leukemia. Biol. Chem. 402, 1547-1564 [Pubmed][Journal] 

Karakitsou, E, Foguet, C., Contreras Mostazo, M.G., Kurrle, N., Schnütgen, F., Michaelis, M., Cinatl, J., Marin, S., and Cascante, M. (2021) Genome-scale integration of transcriptome and metabolome unveils squalene synthase and dihydrofolate reductase as targets against AML cells resistant to chemotherapy. Computational and Structural Biotechnology Journal. doi.org/10.1016/j.csbj.2021.06.049 [Pubmed][Journal] 

Nimbarte, V.D., Wirmer-Bartoschek, J., Gande, S.L., Alshamleh, I., Seibert, M., Nasiri, H.R., Schnütgen, F., Serve, H., and Schwalbe, H. (2021) Fragment-based Lead Discovery and Design of Indole-derived c-Myc Promoter G-Quadruplex Binders to Down-regulate c-Myc Expression in Cancer Cells. ChemMedChem 10.1002/cmdc.202000835 [Pubmed][Journal] 

Malkomes, P., Lunger, I., Oppermann, E., Abou-El-Ardat, K., Oellerich, T., Guenther, S., Canbulat, C., Bothur, S., Schnütgen, F., Yu, W., Wingert, S., Haetscher, N., Catapano, C., Dietz, M.S., Heilemann, M., Kvasnicka, H.-M., Holzer, K., Serve, H., Bechstein, W.O., and Rieger, M. (2021) Transglutaminase 2 promotes tumorigenicity of colon cancer cells by inactivation of the tumor suppressor p53. Oncogene 40, 4352-4367 [Pubmed][Journal] 

Seibert, M., Kurrle, N., Schnütgen, F., and Serve, H. (2021) Amino acid sensory complex proteins in mTORC1 and macroautophagy regulation. Matrix Biol. doi: 10.1016/j.matbio.2021.01.001. [Pubmed][Journal] 

12. Kumar, R., Pereira, R.S., Zanetti, C., Minciacchi, V.R., Merten, M., Meister, M., Niemann, J., Dietz, M.S., Rüssel, N., Schnütgen, F., Tamai, M., Akahane, K., Inukai, T., Oellerich, T., Kvasnicka, H.M., Pfeifer, H., Nicolini, F.E., Heilemann, M., Van Etten, R.A., & Krause, D. (2020) Specific, targetable interactions with the microenvironment influence imatinib-resistant chronic myeloid leukemia. Leukemia 34, 2087-2101[Pubmed][Journal] 

10. Contreras Mostazo, M.G., Kurrle, N., Casado, M., Fuhrmann, D., Alshamleh, I., Häupl, B., Martín-Sanz, P., Brüne, B., Serve, H., Schwalbe, H., Schnütgen, F.*, Marin, S.*, and Cascante, M.* (2020) Metabolic plasticity is an essential requirement of acquired tyrosine kinase inhibitor resistance in Chronic Myeloid Leukemia. Cancers 12, 3443[Pubmed][Journal] 

Kreitz, J., Schönfeld, C., Seibert, M., Stolp, V., Alshamleh, I., Oellerich, T., Steffen, B., Schwalbe, H., Schnütgen, F., Kurrle, N., Serve, H. (2019) Metabolic Plasticity of Acute Myeloid Leukemia. Cells. 8, E805. [Pubmed][Journal] 

Barth, J., Abou-El-Ardat, K., Dalic, D., Kurrle, N., Maier, A.M., Mohr, S., Schütte, J., Vassen, L., Greve, G., Schulz-Fincke, J., Schmitt, M., Tosic, M., Metzger, E., Bug, G., Khandanpour, C., Wagner, S.A., Lübbert, M., Jung, M., Serve, H., Schüle, R. & Berg, T. (2018) LSD1 inhibition by tranylcypromine derivatives interferes with GFI1-mediated repression of PU.1 target genes and induces differentiation in AML. Leukemia. 2019 Jan 24. in press  [Pubmed][Journal] 

Fuhrmann, D., Olesch, C., Kurrle, N., Schnütgen, F., Zukunft, S., Fleming, I., & Brüne, B. (2019) Chronic hypoxia enhances β-oxidation-dependent electron transport via electron transferring flavoproteins. Cells. in press  [Pubmed][Journal] 

Thöne, F.M.B.*, Kurrle, N.*, von Melchner, H., & Schnütgen, F. (2019) CRISPR/Cas9-mediated generic protein tagging in mammalian cells. Methods. 164-165, 59-66  [Pubmed][Journal] 

Sürün, D., von Melchner, H., & Schnütgen, F. (2018) CRISPR/Cas9 genome engineering in hematopoietic cells. Drug Discovery Today: Technologies. Epub 2018 [Pubmed][Journal] 

Namgaladze, D., Zukunft, S., Schnütgen, F., Kurrle, N., Fleming, I., Fuhrmann, D., & Brüne, B. (2018) Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase. Front. Immunol. 9, 2858 [Pubmed][Journal] 

Sürün, D., Schwäble, J., Tomasovic, A., Ehling, R., Stein, S., Kurrle, N., von Melchner, H., and Schnütgen, F. (2018) High Efficiency Gene Correction in Hematopoietic Cells by Donor-Template-Free CRISPR/Cas9 Genome Editing. Mol Ther Nucleic Acids. 10: 1–8. [Pubmed][Journal] 

Atlante, S., Visintin, A., Marini, E., Savoia, M., Dianzani, C., Giorgis, M., Sürün, D., Maione, F., Schnütgen, F., Farsetti, A., Zeiher, A.M., Bertinaria, M., Giraudo, E., Spallotta, F., Cencioni, C. & Gaetano, C. (2018) Α-Ketoglutarate Dehydrogenase Inhibition Counteracts Breast Cancer-Associated Lung Metastasis. Cell Death Dis. 9, 756 [Pubmed][Journal] 

Cencioni, C., Spallotta, F., Savoia, M., Kuenne, C., Guenther, S., Re, A., Wingert, S., Rehage, M., Sürün, D., Siragusa, M., Smith, J.G., Schnütgen, F., Von Melchner, H., Rieger, M.A., Martelli, F. Riccio, A., Fleming, I., Braun, T., Zeiher, A.M., Farsetti, A., & Gaetano, C. (2018) Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells. Nat. Commun. 9. [Pubmed][Journal] 

Fettel, J., Kühn, B., Guillen, N.A., Sürün, D., Peters, M., Bauer, R., Angioni, C., Geisslinger, G., Schnütgen, F., Meyer zu Heringdorf, D., Werz, O., Meybohm, P., Zacharowski, K., Steinhilber, D., Roos, J. & Maier, T.J. (2018) Sphingosine-1-phosphate (S1P) induces potent anti-inflammatory effects in vitro and in vivo by S1P receptor 4–mediated suppression of 5-lipoxygenase activity. FASEB J., fj.201800221R [Pubmed][Journal] 

Spallotta, F., Cencioni, C., Atlante, S., Garella, D., Cocco, M., Mori, M., Mastrocola, R., Kuenne, C., Guenther, S., Nanni, S., Azzimato, V., Zukunft, S., Kornberger, A., Sürün, D., Schnütgen, F., von Melchner, H., Di Stilo, A., Aragno, M., Braspenning, M., van Criekinge, W., De Blasio, M.J., Ritchie, R.H., Zaccagnini, G., Martelli, F., Farsetti, A., Fleming, I., Braun, T., Beiras-Fernandez, A., Botta, B., Collino, M., Bertinaria, M., Zeiher, A.M., & Gaetano, C. (2018) Stable Oxidative Cytosine Modifications Accumulate in Cardiac Mesenchymal Cells from Type2 Diabetes Patients: Rescue by α-Ketoglutarate and TET-TDG Functional Reactivation. Circ. Res. 122, 31–46. [Pubmed][Journal] 

Zhou F, Liu Y, Rohde C, Pauli C, Gerloff D, Köhn M, Misiak D, Bäumer N, Cui C, Göllner S, Oellerich T, Serve H, Garcia-Cuellar MP, Slany R, Maciejewski JP, Przychodzen B, Seliger B, Klein HU, Bartenhagen C, Berdel WE, Dugas M, Taketo MM, Farouq D, Schwartz S, Regev A, Hébert J, Sauvageau G, Pabst C, Hüttelmaier S, Müller-Tidow C. (2017) AML1-ETO requires enhanced C/D box snoRNA/RNP formation to induce self-renewal and leukaemia. Nat Cell Biol. in press [Pubmed][Journal] 

Mohr S*, Doebele C*, Comoglio F*, Berg T*, Beck J, Bohnenberger H, Alexe G, Corso J, Ströbel P, Wachter A, Beissbarth T, Schnütgen F, Cremer A, Haetscher N, Göllner S, Rouhi A, Palmqvist L, Rieger MA, Schroeder T, Bönig H, Müller-Tidow C, Kuchenbauer F, Schütz E, Green AR, Urlaub H, Stegmaier K, Humphries RK, Serve H, Oellerich T. (2017) Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia. Cancer Cell 31, 549-562 [Pubmed][Journal]

Wesely J, Steiner M, Schnütgen F, Kaulich M, Rieger MA, Zörnig M. (2017) Delayed Mesoderm and Erythroid Differentiation of Murine Embryonic Stem Cells in the Absence of the Transcriptional Regulator FUBP1. Stem Cells Int 2017, 5762301 [Pubmed][Journal]

Schneider C, Oellerich T, Baldauf HM, Schwarz SM, Thomas D, Flick R, Bohnenberger H, Kaderali L, Stegmann L, Cremer A, Martin M, Lohmeyer J, Michaelis M, Hornung V, Schliemann C, Berdel WE, Hartmann W, Wardelmann E, Comoglio F, Hansmann ML, Yakunin AF, Geisslinger G, Ströbel P, Ferreirós N, Serve H, Keppler OT, Cinatl J Jr. (2017) SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia. Nat. Med. 23, 250-255 [Pubmed][Journal]

Strassman A, Schnütgen F, Dai Q, Jones JC, Gomez AC, Pitstick L, Holton NE, Moskal R, Leslie ER, von Melchner H, Beier DR, Bjork BC. (2017) Generation of a multipurpose Prdm16 allele by targeted trapping. Dis Model Mech in press [Pubmed][Journal]

Rezende F, Prior KK, Löwe O, Wittig I, Strecker V, Moll F, Helfinger V, Schnütgen F, Kurrle N, Wempe F, Walter M, Zukunft S, Luck B, Fleming I, Weissmann N, Brandes RP, Schröder K. (2017) Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays. Free Radic Biol Med 102, 57-66 [Pubmed][Journal]

Tomasovic A*, Kurrle N*, Wempe F, De-Zolt S, Scheibe S, Koli K, Serchinger M, Schnütgen F, Sürün D, Sterner-Kock A, Weissmann N, von Melchner H. (2017) Ltbp4 regulates Pdgfrβ expression via TGFβ-dependent modulation of Nrf2 transcription factor function. Matrix Biol 59, 109-120 [Pubmed][Journal]

Ball AK, Beilstein K, Wittmann S, Sürün D, Saul MJ, Schnütgen F, Flamand N, Capelo R, Kahnt AS, Frey H, Schaefer L, Marschalek R, Häfner AK, Steinhilber D. (2017) Characterization and cellular localization of human 5-lipoxygenase and its protein isoforms 5-LOΔ13, 5-LOΔ4 and 5-LOp12. Biochim Biophys Acta 1862, 531-571 [Pubmed][Journal]

Völlner F, Ali J, Kurrle N, Exner Y, Eming R, Hertl M, Banning A, Tikkanen R. (2016) Loss of flotillin expression results in weakened desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes. Sci Rep 6, 28820 [Pubmed][Journal]

Lindner S, Berg T, Riemann J, Ajib S, Jedlickova Z, Gueller S, Lang F, Martin H, Serve H, Bacigalupo A, Bug G. (2016) Mismatched unrelated hematopoietic stem cell transplantation with post-transplant cyclophosphamide for high-risk acute myeloid leukemia. Ann Hematol 95,1023-1025 [Pubmed][Journal]

Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, Schnütgen F, Scheibe S, Seimetz M, Jaksch P, Hyman A, Weissmann N, von Melchner H. (2015) Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions. 290, 9735-9752 [Pubmed][Journal]

Jayavelu AK, Müller JP, Bauer R, Böhmer SA, Lässig J, Cerny-Reiterer S, Sperr WR, Valent P, Maurer B, Moriggl R, Schröder K, Shah AM, Fischer M, Scholl S, Barth J, Oellerich T, Berg T, Serve H, Frey S, Fischer T, Heidel FH, Böhmer FD. (2015) NOX4-driven ROS formation mediates PTP inactivation and cell transformation in FLT3ITD-positive AML cells. Leukemia 30, 473-483 [Pubmed][Journal]

Bohnenberger H, Ströbel P, Mohr S, Corso J, Berg T, Urlaub H, Lenz C, Serve H, Oellerich T. (2015) Quantitative mass spectrometric profiling of cancer-cell proteomes derived from liquid and solid tumors. J Vis Exp. 93, e52435 [Pubmed][Journal]

Oellerich T, Mohr S, Corso J, Beck J, Döbele C, Braun H, Cremer A, Münch S, Wicht J, Oellerich MF, Bug G, Bohnenberger H, Perske C, Schütz E, Urlaub H, Serve H. (2015) FLT3-ITD and TLR9 use Bruton tyrosine kinase to activate distinct transcriptional programs mediating AML cell survival and proliferation. Blood 125, 1936-1947 [Pubmed][Journal]

Rabenhorst U, Thalheimer FB, Gerlach K, Kijonka M, Böhm S, Krause DS, Vauti F, Arnold HH, Schroeder T, Schnütgen F, von Melchner H, Rieger MA, Zörnig M. (2015) Single-Stranded DNA-Binding Transcriptional Regulator FUBP1 Is Essential for Fetal and Adult Hematopoietic Stem Cell Self-Renewal. Cell Rep 11, 1847-1855 [Pubmed][Journal]

Berg T, Thoene S, Yap D, Wee T, Schoeler N, Rosten P, Lim E, Bilenky M, Mungall AJ, Oellerich T, Lee S, Lai CK, Umlandt P, Salmi A, Chang H, Yue L, Lai D, Cheng SW, Morin RD, Hirst M, Serve H, Marra MA, Morin GB, Gascoyne RD, Aparicio SA, Humphries RK. (2014) A transgenic mouse model demonstrating the oncogenic role of mutations in the polycomb-group gene EZH2 in lymphomagenesis. Blood 123, 3914-3924 [Pubmed][Journal]

Anastassiadis K, Schnütgen F, von Melchner H, Stewart AF. (2013) Gene targeting and site-specific recombination in mouse ES cells. Methods Enzymol 533, 133-155 [Pubmed][Journal]

Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling MC, Bottomley J, Brown SD, Bürger A, Bult CJ, Bushell W, Collins FS, Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray M, Frendewey D, Friedel RH, Grosveld FG, Hansen J, Hérault Y, Hicks G, Hörlein A, Houghton R, Hrabé de Angelis M, Huylebroeck D, Iyer V, de Jong PJ, Kadin JA, Kaloff C, Kennedy K, Koutsourakis M, Lloyd KC, Marschall S, Mason J, McKerlie C, McLeod MP, von Melchner H, Moore M, Mujica AO, Nagy A, Nefedov M, Nutter LM, Pavlovic G, Peterson JL, Pollock J, Ramirez-Solis R, Rancourt DE, Raspa M, Remacle JE, Ringwald M, Rosen B, Rosenthal N, Rossant J, Ruiz Noppinger P, Ryder E, Schick JZ, Schnütgen F, Schofield P, Seisenberger C, Selloum M, Simpson EM, Skarnes WC, Smedley D, Stanford WL, Stewart AF, Stone K, Swan K, Tadepally H, Teboul L, Tocchini-Valentini GP, Valenzuela D, West AP, Yamamura K, Yoshinaga Y, Wurst W. (2012) The mammalian gene function resource: the International Knockout Mouse Consortium. Mamm. Genome 23, 580-586 [Pubmed][Journal]

Elling U, Taubenschmid J, Wirnsberger G, O'Malley R, Demers SP, Vanhaelen Q, Shukalyuk AI, Schmauss G, Schramek D, Schnuetgen F, von Melchner H, Ecker JR, Stanford WL, Zuber J, Stark A, Penninger JM. (2011) Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. Cell Stem Cell 9, 563-574 [Pubmed][Journal]

Schnütgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, deVries I, Wurst W, Hyman A, Mann M, von Melchner H. (2011) Resources for proteomics in mouse embryonic stem cells. Nat Methods 8, 103-104 [Pubmed][Journal]

Schnütgen F, Ehrmann F, Ruiz-Noppinger P, von Melchner H. (2011) High throughput gene trapping and postinsertional modifications of gene trap alleles. Methods 53, 347-355 [Pubmed][Journal]

Schebelle L, Wolf C, Stribl C, Javaheri T, Schnütgen F, Ettinger A, Ivics Z, Hansen J, Ruiz P, von Melchner H, Wurst W, Floss T. (2010) Efficient conditional and promoter-specific in vivo expression of cDNAs of choice by taking advantage of recombinase-mediated cassette exchange using FlEx gene traps. Nucleic Acids Res 38, e106 [Pubmed][Journal]

De-Zolt S, Altschmied J, Ruiz P, von Melchner H, Schnütgen F. (2009) Gene-trap vectors and mutagenesis. Methods Mol Biol 530, 29-47 [Pubmed][Journal]

Schnütgen F, Hansen J, De-Zolt S, Horn C, Lutz M, Floss T, Wurst W, Noppinger PR, von Melchner H. (2008) Enhanced gene trapping in mouse embryonic stem cells. Nucleic Acids Res 36, e133 [Pubmed][Journal]

Floss T, Schnütgen F. (2008) Conditional gene trapping using the FLEx system. Methods Mol Biol 435, 127-138 [Pubmed][Journal]

Wegmeyer H, Egea J, Rabe N, Gezelius H, Filosa A, Enjin A, Varoqueaux F, Deininger K, Schnütgen F, Brose N, Klein R, Kullander K, Betz A. (2007) EphA4-dependent axon guidance is mediated by the RacGAP alpha2-chimaerin. Neuron 55, 756-767 [Pubmed][Journal]

Horn C, Hansen J, Schnütgen F, Seisenberger C, Floss T, Irgang M, De-Zolt S, Wurst W, von Melchner H, Noppinger PR. (2007) Splinkerette PCR for more efficient characterization of gene trap events. Nat Genet 39, 933-934 [Pubmed][Journal]

De-Zolt S, Schnütgen F, Seisenberger C, Hansen J, Hollatz M, Floss T, Ruiz P, Wurst W, von Melchner H. (2006) High-throughput trapping of secretory pathway genes in mouse embryonic stem cells. Nucleic Acids Res 34, e25 [Pubmed][Journal]

Schnütgen F, De-Zolt S, Van Sloun P, Hollatz M, Floss T, Hansen J, Altschmied J, Seisenberger C, Ghyselinck NB, Ruiz P, Chambon P, Wurst W, von Melchner H. (2005) Genomewide production of multipurpose alleles for the functional analysis of the mouse genome. Proc Natl Acad Sci USA 102, 7221-7226 [Pubmed][Journal]

Hansen J, Floss T, Van Sloun P, Füchtbauer EM, Vauti F, Arnold HH, Schnütgen F, Wurst W, von Melchner H, Ruiz P. (2003) A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. Proc Natl Acad Sci USA 100, 9918-9922 [Pubmed][Journal]

Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, Ghyselinck NB. (2003) A directional strategy for monitoring Cre-mediated recombination at the cellular level in the mouse. Nat Biotechnol 21, 562-565 [Pubmed][Journal]